Tag Archives: pathogens

April 8, 2026

This Company Wants Your Dirty Grow Air Filters

By Pam Chmiel

It is not uncommon for industries outside cannabis to adapt their technologies to address challenges unique to this maturing sector. American Air Filter International (AAF) is one such company, bringing decades of experience in air filtration for critical environments such as pharmaceutical clean rooms, food manufacturing facilities, hospitals, and even jet fuel applications.

According to Nikki Sasher, a microbiologist and head of the Clean Air Laboratory at American Air Filter International, cannabis cultivation facilities face a host of airborne risks, with mold at the top of the list. Once mold enters a grow, the financial consequences can escalate quickly, from destroyed harvests to failed compliance tests. Beyond lost revenue, there are serious biosecurity and worker health concerns tied to inhaling mold spores. In cases where Aspergillus reaches the lower lungs, Sasher notes, the mold can colonize and continue to propagate inside the body.

In 2022, a Trulieve employee died after suffering an asthma attack linked to prolonged exposure to ground cannabis dust in her workplace. Following the incident, OSHA cited the company for failing to implement adequate dust control measures, including proper ventilation and the use of HEPA filtration on vacuum equipment.

Health officials and researchers have since identified a range of respiratory hazards common in cannabis cultivation and processing facilities. These include mold and fungi, pesticides and chemical residues, and terpenes and other volatile organic compounds. Many of these compounds create strong odors and can interact with other airborne agents to form irritants that pose both acute and long-term health risks.

These incidents present a growing safety and occupational health challenge within the rapidly expanding cannabis industry, one that is increasingly difficult to ignore.

Filtration Is Not Yet an Industry-Wide Standard

Despite these risks, comprehensive air filtration is far from universal across cannabis operations. Sasher explains that geography plays a role. Drier climates, such as Nevada, tend to experience fewer mold issues than states like Michigan, Colorado, and New York, where temperature swings and higher humidity create ideal conditions for microbial growth.

Greenhouses, in particular, present ongoing challenges due to constant water activity created by irrigation systems and plant respiration. Sasher frequently sees mold outbreaks occur in drying rooms, where freshly harvested plants retain moisture. If mold is already present in the room or circulating through the air handling system, spores can easily settle onto wet plant material. Drying rooms are typically smaller and more enclosed than other areas of a cultivation facility, making them among the highest-risk environments in the entire operation.

The use of high-tech air filtration systems has only been around for less than twenty years; some sectors still need to catch up to the standards required in food and pharmaceutical manufacturing, such as adhering to minimum efficiency ratings, change-out intervals, locations, first-in, first-out process flow, and hazard analysis.

AAF Wants Your Dirty Filters

To spark greater industry awareness around the benefits of proper air filtration, American Air Filter International has launched a free filter-testing program for cannabis cultivators. Through the initiative, growers are invited to submit used air filters from their facilities, allowing AAF to analyze real-world contamination and build a clearer picture of airborne risks across the sector.

According to Sasher, the filters are evaluated for efficiency, resistance, and pressure drop, as well as downstream mold capture to determine what is circulating in process-facing air. AAF also uses scanning electron microscopy to examine the filter media at the microscopic level, revealing how particulate matter, such as mold spores, dust, and plant material, accumulates and impacts performance over time.

To expand the scope of the research, AAF has partnered with Dr. Alison Justice of the Cannabis Research Center. Together, the organizations are collecting data that will help quantify air quality risks and translate them into practical guidance for cultivators.

Exposed Areas in Cultivation Facilities

Modern cultivation facilities rely on complex climate control systems that include HVAC units, humidifiers, and dehumidifiers, each requiring filters with different efficiency ratings. Sasher notes that many of the filters currently in use fall below recommended standards, a gap AAF hopes to validate through its research partnership with the Cannabis Research Center. The goal is to help growers better understand which efficiency ratings are needed in different parts of the facility to meaningfully reduce risk.

Mold spores, Sasher emphasizes, are ubiquitous. Air filtration is only one engineering control among many, alongside practices such as room fogging and sanitation protocols. Once mold is introduced into a facility, it can be extremely difficult to eliminate.

One uniquely cannabis-specific challenge is the presence of trichomes. These microscopic, sticky particles easily become airborne during cultivation and processing, where they accumulate on filters, restrict airflow, and degrade filtration performance. As part of the testing program, AAF is evaluating how sticky versus non-sticky particulate matter affects filter lifespan and efficiency. The findings could help growers better determine optimal change-out schedules and identify operational factors that accelerate filter failure.

For Sasher, biosecurity is the most compelling reason to invest in proper air filtration. Through its collaboration with Dr. Justice, AAF aims to merge air quality science with cultivation expertise to produce data-driven research that the industry currently lacks. The partnership is focused on developing practical guidance around filtration standards, airflow management, preventative maintenance, and standard operating procedures, areas where formal benchmarks are still largely absent.

Looking ahead, the research team plans to identify pilot cultivation sites where filtration can be evaluated directly within active grow environments. By measuring conditions before and after filtration upgrades, they hope to quantify the impact on contamination levels and overall plant health. Sasher points to similar work in agricultural settings, where improved air filtration reduced livestock mortality rates by double digits, delivering measurable economic gains.

While cannabis-specific results will take time, Sasher believes the approach has the potential to deliver actionable insights that the industry can implement quickly. Even cultivators who are not using AFF products, she says, will be able to apply the findings to strengthen biosecurity, protect workers, and reduce costly crop losses.

Beyond Particulates: Managing Odors and Emissions

While much of the focus in cultivation facilities centers on particulate filtration, Sasher emphasizes that molecular, carbon-based filtration plays an equally important role in cannabis operations. These systems are designed to scrub gases and volatile compounds from outgoing air, an increasingly critical requirement as cultivation and extraction facilities operate closer to residential and commercial neighborhoods, and as consumption lounges come online.

In cannabis, terpenes are responsible for strong odors, as well as gases released during extraction processes that use hydrocarbons such as butane. These gas-phase emissions are subject to strict environmental and safety standards, particularly in regulated markets where operators are required to control odors and limit volatile organic compound emissions.

American Air Filter International addresses this challenge through a dual filtration approach. Pleated particulate filters support plant health and worker safety by capturing airborne contaminants within the facility, while carbon-based molecular filtration systems target gas-phase pollutants before exhaust is released outdoors. Together, these systems help operators remain compliant with local regulations while reducing environmental impact.

Sasher also points to a key differentiator in AAF’s gas-phase filtration program: the ability to test and monitor the remaining life and gas-holding capacity of carbon filters. By measuring remaining adsorption capacity, AAF provides operators with data-driven guidance on when to replace filters. This reduces guesswork, prevents performance failures, and supports more predictable maintenance schedules.

As regulators, communities, and workers place increasing scrutiny on air quality, molecular filtration is becoming a necessary extension of cultivation and extraction infrastructure rather than an option. As the industry inches toward rescheduling and ultimately descheduling, GMP-certified facilities will be required, and with that comes clean-air filtration.

CONTACT NIKKI SASHER IF YOU WOULD LIKE TO PARTICIPATE IN THE FREE AIR FILTER STUDY: nsasher@aafintl.com

Listen to Nikki Sasher’s full interview on the Innovating Cannabis Podcast.

October 15, 2019

Soapbox

Searching for the Good Stuff

By Cindy Rice

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Someone approached me the other day, wanting to know what was the real story about hemp and CBD.

He said he had “a guy” who gave him a CBD salve as part of a study, supposedly “the good stuff,” to help his knee. He couldn’t understand why he was the only one out of 20 people in the group that felt no relief. He happened to have this CBD salve with him, along with a second brand that he hadn’t yet tried. The “good stuff” had slick, colorful packaging, a beautiful logo and powerful marketing messages about the phytocannabinoids and essential oils in the jar. The other CBD product was in a dull grey tin, an ugly duckling, and not nearly so impressive on the outside- I’ll call it “Homer’s Brew.” My friend dismissed Homer’s Brew outright, as not even worth trying. I told him that not all CBD products are created equal, that you can’t always believe the claims on the package, including the cannabinoid potency displayed on the label.

The structure of cannabidiol (CBD), one of 400 active compounds found in cannabis.

I told him to search for the Certificate of Analysis (COA) for each of the two products, specifically, lab test results validating the CBD dosage per serving, and also the breakdown of pesticides, heavy metals and microbials. He had to do a little digging and emailing, as it wasn’t readily available for either company, but the next day, results were in. The “good stuff” with the slick packaging and bold claims had mere trace amounts of CBD, with some hemp and essential oils- no tests for pesticides or contaminants of any kind. Hmmm, no wonder he was disappointed. Homer’s Brew’s COA came in with flying colors – a reputable lab had confirmed safe levels of pesticides, pathogens and heavy metals, and the CBD level was substantial, with a detailed cannabinoid breakdown in the lab report.

In spite of the varying legality of hemp-derived CBD products from one state to the next, consumers are gobbling up costly CBD salves, tinctures and edibles in markets, gyms and online. Like moths to a flame, they are pulled in by the CBD name and lofty promises, not always understanding what they are getting for their money. They trust that these products are safe, licensed, inspected and regulated by some agency, otherwise, “they wouldn’t be on the shelves, would they?”

In spite of the 2018 Farm Bill, FDA still has not recognized the legality of products containing hemp-derived CBD, but some states have gone ahead and given them a green light anyway- check with your own jurisdiction to be sure. In the meantime, hemp-derived CBD products are slipping through the regulatory cracks, depending on the state. It is confusing, for sure, and buyer beware.

Separate yourself from the pack of snake-oil salesmen. Test your products for safety and accurate cannabinoid potency, and make a Certificate of Analysis readily available to your customers. Boldly portray your transparency and belief in the quality of your products through this COA.

Providing this information to consumers is the best path to success- safe, satisfied customers who will refer to their friends and family, and most likely come back for more of your “good stuff.”

November 19, 2018

EVIO Labs Florida Expands Operations

By Aaron G. Biros

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More than a year ago, we sat down with Chris Martinez, co-founder and chief operating officer of EVIO Labs Florida, when he just started getting the laboratory off the ground. In February of 2018, they became Florida’s first ISO accredited cannabis testing lab.

Fast-forward almost a year and EVIO Labs Florida is continuing their expansion in the state, now with locations in Broward County and Gainesville. “We are always looking at opportunities to better serve our clients and the patients of Florida,” says Martinez. “Opening Gainesville within a year of Davie was a goal we set for our team. We knew there was a need and opening Gainesville helped support the continued growth of FL medical marijuana program.” He says that between the two locations, they can now process upwards of 1,400 samples a day.

According to Martinez, much of that expanded throughput is thanks to their partnership with Shimadzu. “Our relationship with Shimadzu is very unique,” says Martinez. “Shimadzu instrumentation allows us to test in parts per billion for accuracy and sensitivity levels that empower us to see deep into the chemical makeup of these medicines. Operating in this space where speed and turnaround times are key, these instruments provide us with a platform to meet 24/48-hour deadlines.” They can now screen for contaminants such as pesticides, heavy metals, residual solvents, mycotoxins, aflatoxins and pathogens using instruments such as HPLC, GC-MS/MS, LC-MS/MS and ICP-MS, all provided by Shimadzu.

While Florida doesn’t currently have a final rule on testing thresholds, there are proposed regulations that would require independent lab testing for medical cannabis products. “Our clients are self-regulating at this time and in favor of the current proposed regulation,” says Martinez. “The proposed regulations will give Florida the most comprehensive and stringent testing regulation in the U.S. and arguably the world.”

For Martinez and the rest of the EVIO Labs Florida team, this is about protecting public health. “Our lab’s main focus is always first and foremost patient safety,” says Martinez. “As the market continues to grow, we continue to innovate through business intelligence software and other technologies to streamline the testing process for our customer’s.”

October 23, 2015

Using LIMS in Cannabis Laboratories

By Aaron G. Biros

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LIMS (laboratory information management systems) is a software-based information management tool that can streamline laboratory workflows, data management, automate repetitive steps, and improve instrumentation efficiency. The cannabis industry’s rapid growth, coupled with fluctuating state regulations, gave rise to a number of cannabis testing laboratories nationwide. Cannabis labs test primarily for potency, but testing regulations for pathogens, pesticides, and other contaminants are on their way to approval in California and Colorado.

limsbud — A dried flower prior to sample-preparation to be used for testing

Founded in 2010, BGASoft developed LIMSABC last year. The cloud-based laboratory informatics system is a platform that can manage all of a cannabis testing laboratory’s operational needs, while providing the tracking and audit trails required by some state’s regulations.

“The recreational and medical cannabis industry is in its infancy and many cannabis laboratories are small operations that need to be very capital efficient as they navigate a rapidly changing regulatory environment,” says Tim Kutz, vice president of business development at BGASoft.

“LIMSABC provides a flexible, modern platform to handle all of a testing laboratory’s operational needs while providing the rigorous tracking and audit trail required by today’s regulations, with the ability adapt to future regulations.”

According to Kutz, LIMS can help cannabis laboratories with bi-directional instrument and automation integration, and automate client reporting to help improve efficiencies and reduce errors. Because the software is cloud-based, the system is accessible through a secure web browser connection from any device.

trichome close up — The fine outgrowths, referred to as trichomes, house the majority of the plant’s resin, which is particularly important for sample-preparation in potency testing

“As legalization efforts advance nationwide, many states are putting in place strict regulatory requirements for the testing and handling of cannabis,” says Kutz. “Many states require or will require testing for pesticide levels, terpenes, cannabinoid levels, moisture, heavy metal, fungi and molds.”

As a result of strict sampling requirements, laboratories must account for all the sample test results from a variety of instruments as well as for every gram of the sample, from receiving it to consumption in testing to disposal.

“These requirements can quickly overwhelm even the most efficient laboratory trying to maintain paper and excel based records,” says Kutz. “LIMS allows laboratory personnel to keep sample and requisition-centric records, track the sample quantity and location, integrate all the test data, provide client reports all while providing an audit trail of each and every step.”

As testing regulations continue to roll out, cannabis laboratories will be required to use information management systems for traceability in compliance with state and local laws.